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. 2011 Apr 29;6(4):e19229. doi: 10.1371/journal.pone.0019229

Figure 2. Hypoxia increases histone H3-K9me2 and G9a, but decreases H3K4me3.

Figure 2

(A, B) H3K9me2 and G9a of primary cortical and hippocampal neurons were detected by Western blot analysis at 24 h and 48 h after hypoxia. β-actin was used as control. (C, D) H3K4me3 was detected by Western blot analysis at 24 h and 48 h after hypoxia. Data are expressed as mean ± SD from four separate experiments. *P<0.05, **P<0.01 as compared with Sham; #P<0.05 as compared with H24 h. (E, F) ChIP and q-PCR analysis were performed with an anti-H3K9me2 antibody and primers amplifying the NEP promoter-1 and promoter-2 regions; the bands illustrate the levels of H3K9me2 in the NEP promoter-1 and promoter-2 regions. Ip represents amplification of total input DNA from whole neurons. H3K9 represents DNA bound to H3K9me2 in the sample. Results from four separate experiments are presented as the ratio of H3K9 to Ip. *P<0.05, **P<0.01 as compared with Sham; Sham, normoxia; H, hypoxia.