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. 2011 Apr 29;6(4):e19195. doi: 10.1371/journal.pone.0019195

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Kanazawa et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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BM-MSCs at passage 5 are induced to differentiate into adipocytes, osteoblasts, and chondrocytes-like cells. Cells analyzed by cytochemical staining with Oil Red-O, Alizarin red, or Safranin-O, respectively.