Table 3. Strains and plasmids used in this study.
Strain or plasmid | Genotype and/or description | Reference or source |
Y. pestis KIM6+ strains: | ||
KIM6+ | wild type | [38] |
ΔhmsS | ΔhmsS::cat | [40] |
ΔhmsT | ΔhmsT::cat | This study |
Δy3730 | Δy3730::kan | This study |
ΔhmsP | ΔhmsP::kan | This study |
Δy0203 | Δy0203::kan | This study |
Δy1612 | Δy1612::kan | This study |
Δy2472 | Δy2472::kan | This study |
Δy2559 | Δy2559::kan | This study |
Δy2909 | Δy2909::kan | This study |
Δy3389 | Δy3389::kan | This study |
Δy3841 | Δy3841::kan | This study |
ΔhmsT Δy3730 | ΔhmsT::cat Δy3730::kan | This study |
ΔhmsT ΔhmsP | ΔhmsT::cat ΔhmsP::kan | This study |
ΔhmsT Δy3730 ΔhmsP | ΔhmsT::cat Δy3730::kan ΔhmsP::tet | This study |
KIM6+ (pCD1-kan) | KIM6+ with pCD1-kan | This study |
KIM6+ ΔhmsT Δy3730 (pCD1-kan) | KIM6+ ΔhmsT::cat Δy3730::kan with pCD1-kan | This study |
Plasmids: | ||
p-hmsT (pCBD26) | hmsT in pCR2.1-TOPO | [41] |
p-y3730 | y3730 in pUC18 | This study |
p-hmsP | hmsP in pUC18 | This study |
p-y0203 | y0203 in pCR2.1-TOPO | This study |
p-y1612 | y1612 in pCR2.1-TOPO | This study |
p-y2472 | y2472 in pUC18 | This study |
p-y2559 | y2559 in pCR2.1-TOPO | This study |
p-y2909 | y2909 in pCR2.1-TOPO | This study |
p-y3389 | y3389 in pCR2.1-TOPO | This study |
p-y3841 | y3841 in pCR2.1-TOPO | This study |
p-M-y3730 | y3730 with mutated GGDEF domain (D331A,E332A) in pUC18 | This study |
pCD1-kan | pCD1 virulence plasmid with kan cassette inserted into yadA | This study |