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. 2011 Apr 29;6(4):e19267. doi: 10.1371/journal.pone.0019267

Table 3. Strains and plasmids used in this study.

Strain or plasmid Genotype and/or description Reference or source
Y. pestis KIM6+ strains:
KIM6+ wild type [38]
ΔhmsS ΔhmsS::cat [40]
ΔhmsT ΔhmsT::cat This study
Δy3730 Δy3730::kan This study
ΔhmsP ΔhmsP::kan This study
Δy0203 Δy0203::kan This study
Δy1612 Δy1612::kan This study
Δy2472 Δy2472::kan This study
Δy2559 Δy2559::kan This study
Δy2909 Δy2909::kan This study
Δy3389 Δy3389::kan This study
Δy3841 Δy3841::kan This study
ΔhmsT Δy3730 ΔhmsT::cat Δy3730::kan This study
ΔhmsT ΔhmsP ΔhmsT::cat ΔhmsP::kan This study
ΔhmsT Δy3730 ΔhmsP ΔhmsT::cat Δy3730::kan ΔhmsP::tet This study
KIM6+ (pCD1-kan) KIM6+ with pCD1-kan This study
KIM6+ ΔhmsT Δy3730 (pCD1-kan) KIM6+ ΔhmsT::cat Δy3730::kan with pCD1-kan This study
Plasmids:
p-hmsT (pCBD26) hmsT in pCR2.1-TOPO [41]
p-y3730 y3730 in pUC18 This study
p-hmsP hmsP in pUC18 This study
p-y0203 y0203 in pCR2.1-TOPO This study
p-y1612 y1612 in pCR2.1-TOPO This study
p-y2472 y2472 in pUC18 This study
p-y2559 y2559 in pCR2.1-TOPO This study
p-y2909 y2909 in pCR2.1-TOPO This study
p-y3389 y3389 in pCR2.1-TOPO This study
p-y3841 y3841 in pCR2.1-TOPO This study
p-M-y3730 y3730 with mutated GGDEF domain (D331A,E332A) in pUC18 This study
pCD1-kan pCD1 virulence plasmid with kan cassette inserted into yadA This study