Figure 1. LIgnase activity of bio-traps after one, four, and thirty weeks in the field.

These lignase assays are based on degradation of the lignin substrate analog L-dihydrophenylalanine (L-DOPA) with 0.3% hydrogen peroxide for peroxidase, and without for phenol oxidase. All assays were performed on fresh beads that had been in the ground 48 hours earlier. Enzyme activities reported as absorbance units per gram bead, and are means of six biological replicates with standard error bars shown, and with significance levels between treatments at each time point (p<0.05) are denoted by an asterisk (*).