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. 2011 Apr 29;6(4):e19293. doi: 10.1371/journal.pone.0019293

Figure 3. Mmu-miR-665 inhibits Abcc3 expression by directly targeting the Abcc3 mRNA 3′-UTR.

Figure 3

(A, B) Mmu-miR-665 represses Abcc3 expression. RAW 264.7 cells were transfected with vehicle or precursors of miR-control or mmu-miR-665, and Abcc3 expression was assessed by qRT-PCR and Western blot. Bar graphs in B show the relative intensity of blots (left panel) from three independent determinations with values represent means ± S.E.M. (C, D) Mmu-miR-665 directly targets the Abcc3 mRNA 3′-UTR. RAW 264.7 cells were transfected with a luciferase or a GFP vector containing the Abcc3 3′-UTR in the presence or absence of mmu-miR-665. Luciferase activity (C) and fluorescent intensity (D) were determined. Values represent means ± S.E.M. (n = 6/group; *P<0.05; **P<0.005; ***P<0.001).