Table 2. Primer sequences (F: forward primer, R: reverse Primer) and FRET probe sequences used for genotyping of CEACAM6 variants.

Polymorphism	Primer sequences	FRET probe sequences
rs10415946	F: AGCCCTGGATGTGTCCACA: AGTCCCTGGGGTCCTCAA	TGGATTTACCCCCAGCAAG-FLLC670-AGGTCACAGAGATGTTTGGGGTCCTAG
rs1805223 = p.Pro42Pro	F: CCACCCTAATGCATAGGTCCA: CGATTCTGTGGCAGGTTGT	GAATCCACGCCATTCAATG-FLLC670-CGCAGAGGGGAAGGAGGTTCTTC
rs4803507	F: GCATCGTTCCTTCCTTTATGTAA: TTTTTCCATAAGTGGAGATCGTT	GAATTCACAACACACCTAAACC-FLLC640-AGTATGTTATCAAGAAAAATACTACTTCCAGCCC
rs4803508	F: CCTGTCCCCCTCACTGTCTA: TTTTTCCATAAGTGGAGATCGTT	CTGCTGAAAGATCCAATCCC-FLLC610-GCCAGGCTGCACAGTATCCTTGGG
rs11548735 = p.Gly239Val	F: TGGTTGAGACTTCAGGGTTGTA: TATGGGCTTGGCACATATAGG	CCCAGATGTCCCCACCAT-FLLC610-TCCCCCTCAAAGGCCAATTACCGTC
rs7246116 = pHis260His	F: TGGTTGAGACTTCAGGGTTGTA: TATGGGCTTGGCACATATAGG	CCTGCCACGCAGCCTCTA-FLLC670-CCCACCTGCACAGTACTCTTGGTTTATCAA
rs2701	F: AAGATGTCAAAACAAGACTCCTCAA: AAGTCCAACTCTGAAAAGGACC	CAAGATAGATCTGACACTCTGTTAAGT-FLLC610-ACCCTCTGAAGCTACTTCTTGTGAAATACT
rs10416839	F: CTTTCAGTTATATGTTGGCTCACTTA: AAAAACACAGCATTATAGATCAACAG	CCAGTGGCAGTTTCCTCTG-FLLC640-TGTAGTCTGAATCAGGTGTACAACTGAGCC

Note: FL: Fluorescein, LC610: LightCycler-Red 610; LC640: LightCycler-Red 640. The polymorphic position within the sensor probe is underlined. A phosphate is linked to the 3′-end of the acceptor probe to prevent elongation by the DNA polymerase in the PCR.