Table 2. Summary of QTL positions and effects.
Trait | Chromosome | Estimated position (cM) | Estimated effect sizea | LOD score at peak | % variance explained by QTL | 2-LOD support interval (cM) | Proximal marker flanking 2-LOD interval (position in kb) | Distal marker flanking 2-LOD interval (position in kb) |
G.mellonella survival | ||||||||
Day 5 | IV | 47 | −0.55 | 4.32 | 19 | 43–51 | cntg-126-39521 (420.7) | cntg-124-54688 (492.1) |
VI | 207 | 0.40 | 4.10 | 18 | 201–221 | cntg-53-38593 (1416.8) | cntg-52-2303 (1534.7) | |
VII | 19 | 0.35 | 3.65 | 17 | 14–32 | cntg-167-30076 (253.1) | cntg-165-13625 (433.7) | |
Day 8 | II | 262 | 0.70 | 4.82 | 20 | 255–271 | cntg-65-149896 (2291.3) | cntg-65-55949 (2385.3) |
Growth | ||||||||
Early, iron-supplemented | II | 65 | 0.12 | 3.70 | 12 | 56–71 | cntg-135-275672 (354.4) | cntg-138-30822 (484.5) |
IV | 35 | 0.45 | 5.45 | 26 | 34–37 | cntg-127-64936 (354.4) | cntg-127-8816 (410.5) | |
VI | 296 | 0.13 | 4.32 | 14 | 286–301 | cntg-51-684909 (1966.4) | cntg-51-460598 (2190.8) | |
Late, iron-supplemented | I | 329 | -0.11 | 3.51 | 11 | 319–341 | cntg-112-222346 (3030.3) | cntg-113-57473 (3219.3) |
IV | 35 | 0.44 | 6.92 | 30 | 34–37 | cntg-127-64936 (354.4) | cntg-127-8816 (410.5) | |
VI | 293 | 0.14 | 5.31 | 19 | 288–313 | cntg-51-684909 (1966.4) | cntg-51-372274 (2279.1) | |
VII | 390 | -0.14 | 5.77 | 20 | 383–396 | cntg-36-61980 (3247.2) | cntg-38-171201 (3530.6) | |
Early, iron-limited | IV | 35 | 0.59 | 13.72 | 44 | 34–37 | cntg-127-64936 (354.4) | cntg-127-8816 (410.5) |
Late, iron-limited | IV | 35 | 0.46 | 9.39 | 38 | 34–37 | cntg-127-64936 (354.4) | cntg-127-8816 (410.5) |
A positive effect size indicates that the A4 allele increases the value of the trait compared to the A91 allele and vice versa. Effect sizes are in the units of the trait (i.e., number of G. mellonella larvae in the case of virulence and cm in the case of growth).