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. 2011 Apr 29;6(4):e19343. doi: 10.1371/journal.pone.0019343

Figure 1. LNCaP and VCaP cells are androgen responsive and harbor ERG and ETV1 rearrangements.

Figure 1

a) Prostate cells were analyzed for ETS rearrangement status by performing PCR using rearrangement specific primer. VCaP cells harbor the TMPRSS-ERG rearrangement whereas LNCaP cells contain rearranged ETV1. VCaP and LNCaP cells express ERG and ETV1 protein respectively, under basal conditions. PC-3 cells do not contain either rearrangement and do not express ERG or ETV1. b) VCaP and LNCaP cells express PSA in response to R1881 treatment. PC-3 cells are not androgen responsive. Cells were treated with 10 nM R1881 for 48 hours and PSA expression was analyzed by real-time qPCR. Results were normalized to actin. * ; p<0.0001, n.s.; not-significant. c) R1881 stimulation results in increased ERG and ETV1 mRNA in VCaP and LNCaP cells respectively, but not in PC3 cells. RNA was isolated from androgen stimulated cells and used to perform real-time qPCR. Data was normalized to the level of gene expression in the absence of androgen. d) ERG and ETV1 proteins are expressed in VCaP and LNCaP cells respectively, but not in PC-3 cells. Androgen stimulation resulted in increased ERG and ETV1 protein in VCaP and LNCaP cells. Prostate cells did not express FLI1 protein under basal or androgen stimulated conditions. MOLT4 was used as a positive control cell-line for FLI1 expression.