Table 2. Bacterial strains and plasmids used in this study.

Materials	Genotype, phenotype or characteristics	Reference or origin
Strains
E.coli DH5α	recA1 endA1 gyrA96 thi1 hsdR17 (rk− mk+) supE44 re1A1	[51]
SM10	thr leu tonA lacy supE recA::RP4-2-Tc::Mu Kmr	[51]
Pseudomonas sp. M18	Wild type, PCA, Plt producer, Apr Spr Gms Kms	[26]
M18ΔP1	ΔphzA1-G1, PhzA1::Gmr	This study
M18ΔP2	ΔphzA2-G2, PhzA2::Gmr	This study
M18ΔG	ΔgacA, gacA::Kmr,	[27]
M18ΔGΔP1	ΔphzA1-G1, PhzA1::Gmr ΔgacA, gacA::Kmr	This study
M18ΔGΔP2	ΔphzA2-G2, PhzA2::Gmr ΔgacA, gacA::Kmr	This study
Plasmids
pBLS	pBluescript II KS+ cloning vector, ColE1 replicon, Apr	This lab
pUCGM	Source of Gmr cassette; Apr, Gmr	Dieter Hass
pME18Tc	Gene replacement vector with multiple cloning sites from pUC18	[52]
pME6015	Pvs1-p15A E. coli-Pseudomonas shuttle vector for translational lacZ fusions and promoter probing, Tetr	Dieter Hass
pME6522	Pvs1-p15A E. coli-Pseudomonas shuttle vector for transcriptional lacZ fusions and promoter probing, Tetr	[47]
pMP1C	341 bp fragment from −340 to +1 upstream phzA1-G1 transcription start site cloned into pME 6522	This study
pMP2C	345 bp fragment from −344 to +1 upstream phzA2-G2 transcription start site cloned into pME 6522	This study
pMP1L	691 bp fragment from −340 to +351 upstream phzA1-G1 translational start site cloned into pME 6015	This study
pMP2L	572 bp fragment from −344 to +228 upstream phzA2-G2 translational start site cloned into pME 6015	This study
pMP1aC	430 bp fragment from −340 to +90 upstream phzA1-G1 transcription start site cloned into pME 6522	This study
pMP1bC	595 bp fragment from −340 to +255 upstream phzA1-G1 transcription start site cloned into pME 6522	This study
pMP1cC	677 bp fragment from −340 to +337 upstream phzA1-G1 transcription start site cloned into pME 6522	This study