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. 2011 Apr 29;6(4):e19468. doi: 10.1371/journal.pone.0019468

Figure 2. Effect of CDK inhibition on AP-1 function.

Figure 2

A) 293T cells were transfected with increasing amounts of a plasmid encoding kinase dead CDK4, together with a construct producing murine TLR7 and an AP-1-luciferase reporter plasmid. Empty pRSV.neo plasmid was added to equalize the total amount of transfected DNA. After 24 hours of stimulation with the TLR7 agonist R848, relative amounts of luciferase activity were measured. B) IL-6 production by B cells stimulated with the agonistic anti-mouse CD40 antibody 1C10 and R848 with or without the CDK inhibitor SU9516 was quantified by IL-6-specific ELISA assay, as described in Methods. C) IL-6 production by purified human peripheral B cells stimulated with R848 for 24 hours with or without the CDK inhibitor SU9516 was monitored by ELISA. These data are each representatives of 3 separate experiments.