Skip to main content
. 2011 Apr 29;6(4):e19495. doi: 10.1371/journal.pone.0019495

Figure 3. Contribution of TAMs to CCL20 production in CRC in mice.

Figure 3

(A, B) Cryosections from mouse CRC induced by MNU and H. pylori or mouse normal colon were stained for CCL20 (red, original magni-fication, ×20). Double immunostaining with anti-mouse CCL20 and F4/80 mAb was performed on cryosections derived from mouse CRC. (C) Co-localization of CCL20 with F4/80 staining (original magnification, ×20). (D) CMT93 was transducted with GFP, and confirmed by flow cytometry. (E) Flow cytometric analysis of CCL20+ cells three weeks after GFP+ CMT93 cells grafting in C57BL/6J mice. (F) mRNA levels of CCL20 for co-culture of mouse peritoneal macrophages with CMT93 cancer cells. AU, arbitrary units. (G) Protein levels of CCL20 for co-culture of mouse peritoneal macrophages with CMT93 cancer cells. Representative data are shown which had been reproduced in at least 2 independent experiments. * p<0.05, ** p<0.01,*** p<0.001, Student's t test.