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. 2011 Apr 29;6(4):e19537. doi: 10.1371/journal.pone.0019537

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Proudfoot et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Substrates containing a Ztn3Z site and a ZcasZ site are recombined by ZFR310. The annotation is as in the previous Figures. The band marked with an asterisk is thought to be single-stranded expression plasmid DNA. (B) Substrates containing a ZcasZ site and Tn3 res site I are recombined when ZFR310 and NM resolvase are co-expressed. (C) A substrate containing an Acas6B site and Tn3 res site I is recombined most efficiently when NM resolvase, ZFR^A320 and ZFR^B320 are co-expressed.