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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Nat Methods. 2011 Apr 10;8(5):424–429. doi: 10.1038/nmeth.1593

Fig. 2. Essential media components for human ES cell survival and proliferation.

Fig. 2

(a) The plots show survival of dissociated H1 ES cells 24 hours after plating into the indicated media (Supplementary Table 1) on Matrigel-coated plates. (*p < 0.05, n = 3, relative to survival in TeSR) (b) The plots show proliferation of cells from (a) 96 hours after plating and culture in the same media with daily media change.(*p<0.05, n=3, relative to proliferation in TeSR) (c) The plots show survival (blue) and proliferation (red) of human ES cells dissociated and plated in the indicated media, 22 h and 129 h after plating, respectively. (*p < 0.05, n = 3). (d) The plots show cell numbers over time of H1 cells maintained in defined media (DMEM/F12, NaHCO3, Insulin, FGF2 and LAA) for multiple passages with or without selenium. 150,000 starting cells were seeded in each passage on day 0, 4 and 7. (e) FACS analysis of OCT4 expression in H1 cells grown in the indicated media for 4 passages. Green peak, OCT4 staining; unshaded peak: mouse IgG control. (f) The plots show cloning efficiency of human foreskin iPS cells29 grown in the indicated defined media, with ROCK inhibitor HA100, in hypoxic conditions (*p < 0.05, n = 3). Similar results were obtained for human ES and other iPS cell lines. (g) The plots show cloning efficiency of ES cells in the indicated media under hypoxic (red) and normoxic (blue) conditions. (*p < 0.05, n = 3). Similar results were obtained from iPS cell lines. (h) The plots show fold expansion of ES (H1) and iPS cells15 cultured under hypoxic conditions in the indicated media. 200,000 cells were plated at each passage.