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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Nat Methods. 2011 Apr 10;8(5):424–429. doi: 10.1038/nmeth.1593

Fig. 5. Derivation of human iPS cells directly from biopsy samples in chemically defined conditions.

Fig. 5

(a, b) The plots show growth (fold change in cell number) of adult fibroblast cells (a) plated onto the indicated plate coating materials in E8-based fibroblast media (see Methods) and counted after 4 days (*p < 0.05, n = 3), or (b) cultured in the indicated media on vitronectin (see Methods) and counted 96 hours after plating (*p < 0.05, n = 3). (c) The micrographs show three fibroblast cell lines derived from skin biopsies in defined fibroblast medium on vitronectin-coated plates. Scale bar = 100 µm. (d) The micrographs show representative iPS colonies obtained by reprogramming fibroblasts from (c) according to the procedure in Figure 4a, in the presence of butyrate. Colonies are shown after multiple passages in E8 (TGFβ). Scale bar = 100 µm. (e and f) FACS analysis of pluripotency markers OCT4 and SSEA4 in iPS cells after 10 passages.