Figure 2.

Effects of BI 2536 on ³H-thymidine uptake in neoplastic mast cells (MC). (A) Cell lines (HMC-1.1, HMC-1.2, C2), (B) primary human neoplastic MC (indolent SM, ISM n=2; aggressive SM, ASM, n=1), and (C) primary canine neoplastic MC (mast cell tumor, MCT) were incubated in control medium or with various concentrations of BI 2536, as indicated at 37°C for 48 h. After incubation, ³H-thymidine uptake was measured. Results are expressed as percent of control. In Figure 2A, results represent the mean±standard deviation from five independent experiments, in B and C, the mean±standard deviation of triplicate experiments are shown.