Figure 3.

Effects of BI2536 on cell cycle distribution in neoplastic mast cells (MC). (A) HMC-1.1 cells, (B) HMC-1.2 cells and (C) C2 cells were incubated in control medium (black bars) or 100 nM BI 2536 (gray bars) at 37°C for 48 h. Thereafter, cell cycle distribution was analyzed by flow cytometry. The percentages of cells in G0/G1-phase, G2/M-phase and S-phase, are shown. Results represent the mean±standard deviation from three independent experiments. (D) HMC-1.1 cells, HMC-1.2 cells and C2 cells were incubated in control medium (left panels) or BI 2536 (10 nM in HMC-1.1; 100 nM in HMC-1.2 and C2; right panel) at 37°C for 48 h. Thereafter, cells were spun on cytospin slides and stained with Wright-Giemsa. Slides were viewed under an Olympus BX50F4 microscope (Olympus, Hamburg, Germany; magnification x 40). Images were prepared using an Olympus DP11 camera and Adobe Photoshop CS2 software version 11.0 (Adobe Systems, San Jose, CA, USA) to adapt brightness and contrast.