Figure 4.

BI 2536 induces apoptosis in neoplastic mast cells (MC). (A) HMC-1.1 cells, HMC-1.2 cells, C2 cells, and (B) primary canine neoplastic MC were incubated in control medium or various concentrations of BI 2536 (as indicated) at 37°C for 48 h and 96 h. The percentages of apoptotic cells and mitotic cells were quantified on Wright-Giemsa-stained cytospin preparations. Results in (A) represent the mean±standard deviation of three independent experiments. Results in (B) are from one experiment. (C) HMC-1.1 cells, HMC-1.2 cells and C2 cells were cultured in control medium (left panel) or with 100 nM BI 2536 (right panel) for 48 h, then apoptotic cells were detected by a TUNEL assay. (D) HMC-1.1 cells and HMC-1.2 cells were cultured in control medium and various concentrations of BI 2536. Western blotting was perfomed using antibodies against cleaved caspases 3, 8, and 9, and β-actin.