Figure 1.

JAK2V617F and TET2 mutational burden during in vitro erythroid expansion. Peripheral blood-derived erythroid cells were expanded in vitro. At each time point, erythroid progenitors (0.5x10⁶ cells) were harvested and their genomic DNAs isolated and JAK2V617F and TET2 mutational burden established. (A) JAK2V617F mutational burden during in vitro erythroid expansion with Standard Error. PV subjects depicted by solid lines represent JAK2V617F-positive PV patients with TET2 mutations; dashed lines represent a group of JAK2V617F-positive PV patients without known TET2 mutations. (B) TET2 mutational burden during in vitro erythroid expansion for a group of JAK2V617F PV patients with TET2 mutations with Standard Error.