Figure 1.

(A) Transduction of CD40-positive cells by targeted vector. CD40-negative HE K293 cells and CD40-positive 293CD40 cells were infected with either Ad5-ff-hCD40L or Ad5-ff-cCD40L at MOI of 10, 10² or 10³ vp/cell. Transduction was indicated by luciferase activity; this was measured from cell lysates and is reported as relative light units. Transduction increased with both vectors in both cell lines in a dose dependent manner; the highest level of transduction as indicated by luciferase activity was seen with the Ad5-ff-hCD40L vector. The values were shown as relative infectivity by taking the level of luciferase at 10 vp/cell as 100% and expressing other readings as relative percentage increase. (B) Incubation of cells with recombinant knob blocked transduction by untargeted virus. Cells were incubated with recombinant knob to block CAR prior to infection with wt virus and luciferase activity was used to measure transduction. In CD40-negative cells infected with wt virus, luciferase activity was reduced to 2% while in CD40-positive cells infected with targeted virus, there was little reduction in transduction efficiency as determined by luciferase activity. Error bars indicate standard deviation. *p < 0.05; ***p < 0.001.