Figure 5.

CD40-targeted transduction in primary cells. Three primary cultures of cells obtained from lymph nodes of lymphoma-affected dogs were infected within 24 h of collection with untargeted virus alone (control), untargeted virus and sCAR-CD40L, Ad5-ff-hDC40L or Ad5-ff-cCD40L and luciferase activity was measured from cell lysates. The values were shown as relative infectivity by taking the level of luciferase expression of Ad5 transduced cells as 100% and expressing other readings as relative percentage change. Error bars indicate standard deviation. ***p < 0.001.