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Decreased integrin-mediated phosphorylation of FAK by CD43 expression. CD43-GFP or CD43W277-GFP was expressed in α4-HE K293T cells. These transfectants and α4-HE K293T cells were trypsinized, washed and re-plated onto CS1-coated dishes and incubated for indicated time and then, lyzed. Cellular lysates were subjected to SDS-PAGE and immunoblotting with anti-phospho-FAK [pY397] Ab or anti-FAK mAb.
