Skip to main content
. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Cell. 2011 Apr 15;145(2):284–299. doi: 10.1016/j.cell.2011.03.016

Figure 6. Thorase Deficiency Enhances AMPAR Currents and LTP and Impairs LTD Without Affecting Basal Synaptic Transmission at CA1 Hippocampal Synapses.

Figure 6

(A) Thorase KO CA1 neurons exhibit normal basal synaptic transmission. The amplitude of the fiber volley is plotted against fEPSP slopes. The input/output curves for neurons in slices from WT (n = 8 slices, 8 mice) or KO (n = 6 slices, 6 mice) were not significantly different (p > 0.05, Student’s t-test).

(B) Thorase KO does not affect paired-pulse facilitation (PPF) (WT, n = 8 slices, 6 mice; KO, n = 12 slices, 8 mice) (p > 0.05, Student’s t-test).

(C) Thorase is necessary for LTD induction. LTD was induced by 1 Hz stimulation in slices from WT mice, but not in slices from Thorase KO mice.

(D) Thorase is a negative regulator of LTP. LTP was induced by a tetanic train (100 Hz, 1 s). The magnitude of LTP for slices from KO mice was significantly greater than that for WT mice (KO, n = 8 slices from 4 mice; WT, n = 6 slices from 3 mice. p < 0.01, Student’s t-test).

(E) NMDA and AMPA currents recorded at 40 mV and −70 mV, respectively, in CA1 neurons of slices from WT mice that were (a) untreated, (b) treated with AP-5, (c) treated with AP-5 plus CNQX or (d) treated with AP-5 plus CNQX followed by a 30 minute washout.

(F) NMDA and AMPA currents recorded in slices from (a) WT and (b) KO mice. EPSC input/output relations recorded in slices from (c) WT and (d) KO mice. (e) Histogram of averaged AMPA/NMDA ratio (n = 6; *p < 0.05, Student’s t-test).

(G) I-V curves from WT (thin line) and KO (thick line) mice. Values are the mean of measurements made in 8–10 neurons in 8–10 slices. p < 0.05, Student’s t-test.

(H) I-V curves for slices from WT and KO mice incubated in the presence of AP-5 (8–12 neurons in 8–10 slices). p < 0.05, Student’s t-test.

(I) The rectification index measured by dividing the EPSC amplitude at +40 mV by that at −70 mV shows no significant difference between WT and KO mice.

(J and K) AMPA currents of CA1 pyramidal neurons in brain slices from KO mice are restored to normal by lentiviral-mediated overexpression of Thorase (8–12 neurons in 8–10 slices). *p < 0.05, Student’s t-test compared to the GFP and mAB-Thorase-GFP values.

See also Figure S4.