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Flow SPR for the binding of (A) MP-Ab2-PSA to covalently immobilized Ab1 on the SPR sensor surface and (B) influence of PSA concentration on maximum SPR signals (less zero PSA control) for the binding of MP-Ab2-PSA (●) and SP-Ab2-PSA (▲) conjugates made from different PSA concentrations in calf serum (as denoted). The zero PSA signal results mainly from NSB.
