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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Bioorg Med Chem. 2010 Jun 18;19(3):1106–1114. doi: 10.1016/j.bmc.2010.06.026

Table 1.

Initial and final ratios of reactants in the conjugation reactions and immunoreactivity of the antibody

Initial (EuL/P)i Final (EuL/P)f (R)f/(R)i Immunoreactivitya of 3G4,%
Eu-1 – 3G4 (1) 100 10.1 0.10 17 ± 2
Eu-1 – 3G4 (2) 50 7.4 0.15 88 ± 2
Eu-1 – HSA 20 5.6 0.28 -
Eu-2 – HSA 100 5.9 0.06 -
a

average of duplicate experiments (ELISA assay)