Skip to main content
. Author manuscript; available in PMC: 2012 Jun 22.
Published in final edited form as: Biochemistry. 2010 Dec 22;50(2):287–299. doi: 10.1021/bi1014776

Figure 5. Detection of malonyl–S-LovF by PPant ejection.

Figure 5

The FT-ICR-MS2 of ACP active site containing peptide following incubation of holo-LovF with 1 mM malonyl-CoA. Both pantetheine and phosphopantetheine ejection ion with a mass of 347.127 Da corresponding to the addition of malonyl was detected. The post-ejection charge-loss parent phosphopeptide (MS1 17+ to MS2 16+) was detected at 838.430 m/z (13,398.90 Da). FT-ICR-MS2 of the ACP active site containing peptide following incubation of holo-LovF with low concentrations (<0.1 mM) of malonyl-CoA resulted in the detection of a pantetheine ejection ion at 303.137 m/z corresponding the presence of an acetyl-intermediate.