Table 2.
Viral expression cassettes for in vivo manipulation and detection of astrocyte and interneuronal signaling.
| Protein expressed | Goal | Targeting strategies | References |
|---|---|---|---|
| Channelrhodopsin 2 | Light-mediated cell depolarization | Arenkiel et al. (2007); Gradinaru et al. (2009) | |
| Halorhodopsin | Light-mediated cell hyperpolarization | (i) Cre-lox | Zhang et al. (2007); Chow et al. (2010) |
| Archaerhodopsin-3 | (ii) Cell type – specific promoters | ||
| hM3Dq (G-coupled protein receptor for cell activation) | Drug-based (clozapine-N-oxide) regulation of activity | (iii) Cell type – trophic viruses | Alexander et al. (2009) |
| TRPV1 | Ligand-based (capsaicin) regulation of activity | Arenkiel et al. (2008) | |
| Tn-XXL or GCaMP3 (Fluorescent protein based Ca2+ sensors) | Cell-specific detection of intracellular calcium increases | Mank et al. (2008); Tian et al. (2009) |