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. Author manuscript; available in PMC: 2011 May 2.
Published in final edited form as: J Invest Dermatol. 2010 Nov 25;131(3):688–697. doi: 10.1038/jid.2010.351

Figure 2. Increased Toll-like receptor 2 (TLR2) results in increased kallikrein 5 (KLK5) expression in keratinocytes.

Figure 2

(a–d) TLR2 was overexpressed in normal human epidermal keratinocytes (NHEKs) by culture in the presence of increasing multiplicities of infection (MOIs) of adenovirus vector expressing TLR2 or adeno-null vector for 48 hours. (a) KLK5 mRNA and (d) TLR2 mRNA as measured by quantitative real-time PCR (qPCR). (b) KLK5 protein in the culture media measured by ELISA. (c) Protease activity of the culture media measured by zymography. *P<0.05, **P<0.01, ***P<0.01. (e) NHEKs were cultured in the presence of adenovirus vectors for 48 hours. The expression of TLR2 and KLK5 was examined by immunocytofluorescence, and nuclei were visualized with 4,6-diamidino-2-phenylindole (DAPI). Scale bar = 20 μm.