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. Author manuscript; available in PMC: 2012 Apr 12.
Published in final edited form as: Biochemistry. 2011 Mar 18;50(14):3025–3033. doi: 10.1021/bi101832w

Figure 2. SDS-PAGE analysis of hNTAN1 following tandem affinity purification.

Figure 2

1, hNTAN1 was expressed with C-terminal tandem FLAG-strepII affinity tag from pNTANFLAGSII and purified as described in detail in “Experimental Procedures”. Equivalent product homogeneity was observed following analogous purification of hNTAN1 expressed from pNTAN2FLAGSII (Figure S4). 2, M.W. marker.