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. Author manuscript; available in PMC: 2012 Apr 12.
Published in final edited form as: Biochemistry. 2011 Mar 18;50(14):3025–3033. doi: 10.1021/bi101832w

Table 3. Effects of divalent metals, EDTA, and D-desthiobiotin on hNTAN1 activity.

hNTAN1 (20nM) was preincubated in 50mM Tris-HCl, 150mM NaCl, pH 7.5 at 4°C for 3 hr with either EDTA (1mM), D-desthiobiotin (3mM), or a divalent salt (1mM). Reactions were initiated by addition of the preincubated enzyme (1nM) to 100μM N1-AII in 50mM Tris-HCl, 150mM NaCl, pH 7.5 at 37°C. After 4 min, reactions were quenched and then analyzed by HPLC as described in “Experimental Procedures”.

Effector % Activity Remaining
MgCl2 100
CaCl2 100
CoCl2 58 ± 2.8
MnSO4 40 ±14
NiSO4 39 ± 4.2
CuCl2 0 *
ZnSO4 0
EDTA 100
D-desthiobiotin 100
*

Area of the product peak was less than or equal to that observed in an HPLC trace of N1-AII only at the equivalent reaction conditions.