Table 1.
Nutrient screening using a Plackett-Burman design.
| Variable | Levels (mg/g dry substrate) | ||
|---|---|---|---|
| Nutrient code | Nutrient | Low (−1) | High (+1) |
| A | Yeast extract | 1.0 | 5.0 |
| B | Beef extract | 5.0 | 15.0 |
| C | MnSO4·7H2O | 10.0 | 50.0 |
| D | K2HPO4 | 2.0 | 7.0 |
| E | Soya bean cake | 40.0 | 80.0 |
| F | MgSO4·7H2O | 0.2 | 1.2 |
| G | NH4Cl | 1.0 | 3.0 |
| H | KCl | 0.5 | 1.5 |
| J | (NH4)2HPO4 | 5.0 | 30.0 |
| K | NH4NO3 | 5.0 | 10.0 |
| L | ZnSO4·7H2O | 10.0 | 50.0 |
| M | (NH4)2SO4 | 6.0 | 10.0 |
| N | Corn steep liquor | 40.0 | 80.0 |
| O | Peptone | 5.0 | 15.0 |
| P | Dextrose | 10.0 | 30.0 |
| Q | FeSO4·7H2O | 0.05 | 2.0 |
| R | KH2PO4 | 10.0 | 60.0 |
| S | Urea | 10.0 | 30.0 |