Table 4.

Central composite design (CCD) of factors in coded levels with enzyme activity as response.

Run no.	X1	X2	X3	X4	Inulinase activity (U/g dry substrate)
					Experimental	Predicted
1	−1	1	−1	−1	32.25	29.92
2	1	1	1	1	19.38	24.17
3	1	1	−1	−1	25.78	25.83
4	0	0	−2	0	41.94	35.42
5	−1	1	−1	1	40.83	47.50
6	0	0	0	0	58.43	59.43
7	0	0	0	0	60.75	59.43
8	−1	−1	−1	1	54.69	55.25
9	0	0	0	0	59.23	59.43
10	1	1	−1	1	15.32	12.92
11	2	0	0	0	23.85	26.08
12	1	−1	1	−1	44.89	47.83
13	−1	−1	−1	−1	23.92	28.17
14	1	−1	−1	1	14.37	24.67
15	1	−1	1	1	50.03	42.92
16	−2	0	0	0	45.18	42.75
17	−1	1	1	−1	25.30	24.67
18	0	0	0	0	60.34	59.43
19	−1	−1	1	1	45.59	55.50
20	0	2	0	0	19.42	23.42
21	−1	1	1	1	45.69	40.75
22	−1	−1	1	−1	36.80	29.92
23	1	−1	−1	−1	31.86	28.08
24	0	0	0	2	46.05	37.25
25	1	1	1	−1	48.29	38.58
26	0	0	0	0	57.44	59.43
27	0	−2	0	0	45.30	40.42
28	0	0	0	0	60.23	59.43
29	0	0	2	0	42.40	48.42
30	0	0	0	−2	16.42	24.58