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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Eur J Immunol. 2011 Apr 12;41(5):1465–1479. doi: 10.1002/eji.201041241

Figure 6. Detection of natural RTL-like two-domain MHC class II molecules in MS subject serum and plasma samples.

Figure 6

A) ELISA binding assay of purified 1B11 Fab to immobilized RTLs and four-domain recombinant MHC complexes. * indicates recombinant complexes that were compared only with RTL1000. Data are representative of three independent experiments B) Fab 1B11 detected RTL-like MHC class II material in serum and plasma samples from MS subjects and a pool of 3 healthy controls. Serum or plasma was collected prior to infusion of RTL1000 from MS Subjects #03–302, #04–402, #24, #40, #42 & #44 (0 time), from Subject #42 at 30min after initiating infusion of 200mg RTL1000; and #44 at 120min after initiating infusion of 100mg RTL1000. Differences between the samples and background were evaluated using two-tailed unpaired t-test. *p<0.05; **p<0.01; ***p<0.001.

Data are representative mean + SD of at least three independent experiments..