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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Eur J Immunol. 2011 Apr 12;41(5):1465–1479. doi: 10.1002/eji.201041241

Figure 7. Detection of RTL1000 in plasma of treated MS subjects.

Figure 7

A) Fab 2E4 detected RTL1000 in plasma samples from MS Subjects (#04–402, #03–302, #24, #40, # 42 and #44) before (0 min) and after infusion of the drug and circulating RTL1000 levels were discriminated from native RTL-like material by Fab 1B11. Differences between pre- and post-infusion samples of each subject were evaluated using two-tailed paired t-test. Right panel: Standard curves of various concentrations of RTL1000 and RTL340 that were used for calculation of RTL and RTL-like material concentrations in serum and plasma samples. The minimal thresholds for RTL detection were 12ng/ml for Fab2E4 and 0.1ng/ml for Fab1B11. B) Fab 2E4 detection of RTL1000 by ELISA in plasma of MS subject #42 collected during 120min of infusion of the drug and the following 60 min. Time from the beginning of RTL1000 infusion and the time after completion of the infusion are indicated by brackets. The completion of RTL1000 infusion is indicated by a dashed line. Differences between pre- and post-infusion samples of each subject were evaluated using two-tailed paired t-test. Data in A–B are representative mean + SD of at least three independent experiments. *p<0.05; **p<0.01; ***p<0.001.