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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Eur J Immunol. 2011 Apr 12;41(5):1465–1479. doi: 10.1002/eji.201041241

Figure 8. Binding characterization of G3H8 and D2 Fabs.

Figure 8

A) ELISA of purified anti-G3H8 with immobilized DR4/GAD-555-567, control complex DR4/HA-307-319, GAD-555-567 peptide, and HA-307-319 peptide. Anti-DR mAb (L243) was used to determine the correct conformation and stability of the bound complexes during the binding assay. B) Flow cytometry analysis of Fab G3H8 binding to Preiss APCs pulsed with GAD-555-567 peptide or the control peptides: InsA-1-15, CII-261-273, and HA-307-319. C) ELISA binding of anti-RTL2010 (DR4/GAD-555-567) TCRL (D2), anti-full-length DR4/GAD-555-567 TCRL (G3H8) and anti-MHC II (TU39, BD) to immobilized RTL2010 and full length DR4/GAD-555-567 complexes. Data in A–C are representative of at least three independent experiments.