Table 2.
Data from 85 microsatellites used to genotype the 60 carioca accessions (cream-striped grain type). Of the 85 SSRs screened, 20 were genotyped using a fluorescence technique (*).
| No | SSRs | Motif | Ta** | Allele range (bp) | Number of alleles | PIC** | DP** |
|---|---|---|---|---|---|---|---|
| 01 | SSR-IAC01* | (CT)8 | 56 | 240–262 | 2 | 0.43 | 0.38 |
| 02 | SSR-IAC05 | (TG)6(GA)5 (AG)10 (ACA)5 | 50 | 164–166 | 2 | 0.10 | 0.10 |
| 03 | SSR-IAC09 | (CA)9C (CA)2(TA)6 | 56 | 160–168 | 2 | monomorphic | - |
| 04 | SSR-IAC10 | (GA)12(AG)6 (AG)6 | 56 | 176–188 | 4 | 0.68 | 0.47 |
| 05 | SSR-IAC11 | (GA)24 | 56 | 186–204 | 4 | 0.60 | 0.58 |
| 06 | SSR-IAC13 | (GA)10A (GA)4GG (GA)9 | 56 | 180 | 1 | monomorphic | - |
| 07 | SSR-IAC14* | (GT)7 | 56 | 226–256 | 5 | 0.30 | 0.32 |
| 08 | SSR-IAC16 | (GA)8 | 56 | 220–224 | 3 | 0.29 | 0.32 |
| 09 | SSR-IAC18* | (GT)8 | 56 | 270–300 | 3 | 0.59 | 0.61 |
| 10 | SSR-IAC20 | (GA)7AA (GA)2 | 56 | 182 | 1 | monomorphic | - |
| 11 | SSR-IAC21 | (AC)6 | 56 | 138–140 | 2 | 0.40 | 0.20 |
| 12 | SSR-IAC22 | (TA)8(GA)9 | 56 | 146–148 | 2 | 0.09 | 0.11 |
| 13 | SSR-IAC24 | (AC)7(AT)6 | 56 | 166–168 | 2 | 0.06 | 0.06 |
| 14 | SSR-IAC25 | (CA)6CAA (CA)2 CAA(CA) 3CG (CA)5 | 56 | 260–300 | 3 | 0.49 | 0.12 |
| 15 | SSR-IAC27 | (GT)5 | 56 | 260–278 | 2 | 0.11 | 0.16 |
| 16 | SSR-IAC28 | (GT)5(TC)10(TA)14 | 56 | 280 | 1 | monomorphic | - |
| 17 | SSR-IAC29 | (GA)23 | 56 | 58–158 | 2 | 0.10 | 0.23 |
| 18 | SSR-IAC32* | (TG)7 (TA)6 | 56 | 62–80 | 3 | 0.48 | 0.35 |
| 19 | SSR-IAC34 | (GA)12 | 56 | 180–182 | 2 | 0.49 | 0.52 |
| 20 | SSR-IAC35 | (CT)5 | 56 | 240–242 | 2 | 0.50 | 0.56 |
| 21 | SSR-IAC45 | (TG)5 | 56 | 202 | 1 | monomorphic | - |
| 22 | SSR-IAC46 | (CA)7 | 56 | 220–260 | 4 | 0.63 | 0.31 |
| 23 | SSR-IAC47* | (GA)20 | 56 | 300–330 | 4 | 0.56 | 0.52 |
| 24 | SSR-IAC49 | (AG)9 | 56 | 228–230 | 2 | 0.13 | 0.21 |
| 25 | SSR-IAC51 | (GA)5 CA (GA)9 CA (GA)2 | 56 | 150–160 | 2 | 0.25 | 0.38 |
| 26 | SSR-IAC52 | (GA)11 | 56 | 221–225 | 3 | 0.56 | 0.56 |
| 27 | SSR-IAC53 | (GA)9 | 56 | 164–168 | 3 | 0.52 | 0.12 |
| 28 | SSR-IAC54 | (AC)6 CAAA (TA)3 C (AT)5 | 56 | 110–112 | 2 | 0.09 | 0.09 |
| 29 | SSR-IAC55 | (GA)13 | 56 | 194–202 | 3 | 0.52 | 0.53 |
| 30 | SSR-IAC56* | (AC)8 | 56 | 270–300 | 3 | 0.37 | 0.36 |
| 31 | SSR-IAC57 | (GT)5 | 56 | 280 | 1 | monomorphic | - |
| 32 | SSR-IAC58 | (TG)10 | 56 | 184 | 1 | monomorphic | - |
| 33 | SSR-IAC59* | (AC)7 | 61 | 35–170 | 3 | 0.55 | - |
| 34 | SSR-IAC62 | (AG)14 | 45.3 | 198–210 | 4 | 0.67 | 0.67 |
| 35 | SSR-IAC63 | (AC)6 | 59.8 | 210 | 1 | monomorphic | - |
| 36 | SSR-IAC64* | (AC)6 | 56 | 270–290 | 4 | 0.53 | 0.57 |
| 37 | SSR-IAC65 | (TG)5 | 60 | 270–272 | 2 | 0.10 | 0.10 |
| 38 | SSR-IAC66 | (GA)10 | 56 | 136–144 | 3 | 0.49 | 0.73 |
| 39 | SSR-IAC67 | (GT)7 | 56 | 110 | 1 | monomorphic | - |
| 40 | SSR-IAC68 | (CT)8 | 56 | 260–272 | 4 | 0.53 | 0.82 |
| 41 | SSR-IAC70 | (AC)8 | 60 | 186–188 | 2 | 0.48 | 0.48 |
| 42 | SSR-IAC73 | (AT)6(GT)6 | 60 | 198–230 | 3 | 0.53 | 0.34 |
| 43 | SSR-IAC77 | (CA)6(CT)4 | 60 | 188–190 | 2 | 0.44 | 0.45 |
| 44 | SSR-IAC83 | (TC)11 | 45 | 250–260 | 3 | 0.61 | 0.63 |
| 45 | SSR-IAC87 | (AC)9 | 63.5 | 220–240 | 3 | 0.41 | 0.29 |
| 46 | SSR-IAC88 | (CA)7(AT)7 | 60 | 210–220 | 3 | 0.52 | 0.53 |
| 47 | SSR-IAC91 | (AC)3(TC)2 | 60 | 200–210 | 2 | 0.06 | 0.06 |
| 48 | SSR-IAC96 | (CA)5(TA)2 | 60 | 254–258 | 2 | 0.47 | 0.48 |
| 49 | SSR-IAC97 | (AC)3(TC)2 | 60 | 240 | 1 | monomorphic | - |
| 50 | SSR-IAC98 | (CT)8(TA)3(TG)8 | 60 | 230–290 | 3 | 0.60 | 0.65 |
| 51 | SSR-IAC100 | (AT)4(GT)8 | 60 | 206–210 | 2 | 0.09 | 0.17 |
| 52 | SSR-IAC101 | (AC)7 | 60 | 186–190 | 2 | 0.29 | - |
| 53 | SSR-IAC102 | (CT)7 GTCA (CT)8 | 60 | 176–178 | 2 | 0.39 | 0.42 |
| 54 | SSR-IAC127 | (TA)3 T (TGA)3 G (TA)3 | 63.3 | 168–170 | 2 | 0.50 | 0.50 |
| 55 | SSR-IAC128 | (AC)7 GGA (TC)2 | 56.7 | 168–190 | 2 | 0.31 | 0.35 |
| 56 | SSR-IAC129 | (TG)2 G (CT)2 TCT (GA)2 | 56.7 | 250–258 | 2 | 0.47 | 0.53 |
| 57 | SSR-IAC134 | (AC)6 | 56.7 | 218–250 | 2 | 0.41 | 0.39 |
| 58 | SSR-IAC136 | (CA)7 (AT)5 | 56.7 | 240–270 | 2 | 0.43 | 0.17 |
| 59 | SSR-IAC141 | (TCT)3 A (CT)13 | 59.4 | 214–218 | 2 | 0.40 | 0.46 |
| 60 | SSR-IAC143* | (TC)2 T (TC)2 T (TC)2 | 63.3 | 170–200 | 4 | 0.51 | 0.49 |
| 61 | SSR-IAC144* | (CT)10 | 56.7 | 170–220 | 4 | 0.70 | 0.49 |
| 62 | SSR-IAC147 | (CA)5 | 56.7 | 230–240 | 2 | 0.46 | 0.42 |
| 63 | SSR-IAC155 | (AG)9 | 56.7 | 196–200 | 2 | 0.04 | 0.01 |
| 64 | SSR-IAC156 | (TC)3 TG (GC)2 | 56.7 | 230 | 1 | monomorphic | - |
| 65 | SSR-IAC159 | (AC)6/(AC)4 C (CT)2 | 56.7 | 284–296 | 2 | 0.29 | 0.42 |
| 66 | SSR-IAC160 | (TG)2 (TA)2 (TG)5 | 56.7 | 170–174 | 2 | 0.44 | 0.49 |
| 67 | SSR-IAC166 | (CA)2 AA (AC)3/(TA)2 GAC (TG)3 | 56.7 | 186–190 | 2 | 0.05 | 0.36 |
| 68 | SSR-IAC167 | (TG)7 (CG)3 | 56.7 | 138–168 | 2 | 0.34 | 0.31 |
| 69 | SSR-IAC174 | (AT)3 A (AT)2 (AC)7 TTT (CA)3 | 53.2 | 140 | 1 | monomorphic | - |
| 70 | SSR-IAC179 | (AC)2 CTTT (AC)2 CTA (TC)5 | 63.3 | 180–186 | 2 | 0.48 | 0.53 |
| 71 | SSR-IAC180 | (AC)3 T (CA)3 TAA/ (AC)3(AC)3 G (CA)2 | 63.3 | 206 | 1 | monomorphic | - |
| 72 | SSR-IAC181 | (AT)2 AC (AT)3/(AG)5 TAA (AG)2 C (AG)2 | 58.4 | 120 | 1 | monomorphic | - |
| 73 | SSR-IAC183 | (AG)18 A (AC)4 | 56 | 190–196 | 2 | 0.27 | 0.34 |
| 74 | SSR-IAC209 | (AC)2 (TG)3 | 56.7 | 198–200 | 2 | 0.48 | |
| 75 | SSR-IAC211 | (CA)10 (TA)8 | 43.8 | 176 | 1 | monomorphic | - |
| 76 | SSR-IAC226* | (TG)8 | 60 | 240–260 | 4 | 0.56 | 0.65 |
| 77 | SSR-IAC239* | (AG)15 | 60 | 260–300 | 6 | 0.61 | 0.62 |
| 78 | SSR-IAC240* | (CT)10 | 60 | 196–210 | 4 | 0.64 | 0.62 |
| 79 | SSR-IAC242* | (AT)2 (GT)3 | 60 | 256–300 | 2 | 0.36 | 0.38 |
| 80 | SSR-IAC244* | (TC)9 | 60 | 200–226 | 5 | 0.27 | 0.27 |
| 81 | SSR-IAC251* | (AC)11 (AT)12 | 45 | 144–296 | 5 | 0.69 | 0.53 |
| 82 | SSR-IAC272* | (CA)6 | 60 | 200–236 | 4 | 0.42 | 0.45 |
| 83 | SSR-IAC390* | (GT)4 AT (GT)3 | 60 | 190–250 | 5 | 0.64 | 0.62 |
| 84 | FJUNA 167* | (AT)4 AG (GT)6/(AT)4 (GT)6 | 60 | 290–310 | 4 | 0.41 | 0.41 |
| 85 | FJUNA 384* | (CA)5 | 60 | 160–206 | 3 | 0.67 | 0.38 |
**
Ta- annealing temperature; PIC – polymorphism information content; DP – Discrimination power.