Table II.
Characterization of fluorescein and thrombin emulsions. Thrombin precipitation, during the second emulsification step, and subsequent inactivation of thrombin during ADV was suspected in T2.
| Formulation | Mean diameter (μm) | Percent of droplets > 6 μm diameter |
Encapsulation (%) | Volume percent vaporized | Percent change in ACT upon ADV | |
|---|---|---|---|---|---|---|
| Number | Volume | |||||
| F2 | 2.4 ± 0.2 | 2.6 ± 1.8 | 32.3 ± 15.4 | 3.8 ± 1.1 | 100 c | NA |
| T1 | 1.6 ± 0.1 | 0.04 ± 0.02 | 6.7 ±3.0 | 76.3 ± 3.4 | 10.2 ± 4.2 | −7.4 ± 1.6 |
| T2 | 2.6 ± 0.2 | 6.5 ± 1.4 | 83.2 ± 1.0 | 63.7 ± 22.9 a | 25.6 ± 7.1 | 63.0 ± 12.2 a,b |
| T3 | 27.4 ± 5.9 | 89.2 ± 1.1 | 99.9 ± 0.1 | 97.5 ± 0.6 | 100c | −78.0 ± 13.5 b |
| T4 | 3.5 ± 0.1 | 15.5 ± 0.8 | 89.4 ± 4.5 | 98.8 ± 1.1 | 28.7 ± 10.2 | −23.3 ± 5.9 b |
| T5 | 3.9 ± 0.1 | 17.6 ± 0.5 | 88.9 ± 3.1 | 97.2 ± 0.5 | 34.3 ± 15.9 | −19.0 ± 4.9 b |
a
T2 parameters that are potentially affected by thrombin precipitation
b
Statistically significant differences for the percent change in ACT upon ADV
c
Cases where complete vaporization was observed, and thus the number of droplets remaining in solution after ADV was below the noise threshold of the Coulter counter