Figure 7. Combination αCTLA-4/α4-1BB therapy induces higher tumor infiltration by CD4+KLRG1+ T-cells than α4-1BB alone.

Mice challenged with 1.5×10⁵ B16-BL6 cells and treated on days 3, 6 and 9, were sacrificed on Day 15. The number of KLRG1+ T-cells of a given lineage was calculated by multiplying the %KLRG1+ TIL determined by flow cytometry by the measured number of CD45+ lymphocytes per mm³ of tumor. Data shown are ratios of the absolute number of KLRG1+ T-cells from combination treated tumors to the number of KLRG1+ T-cells from α4-1BB alone treated tumors. Ratios were calculated for 5 independent experiments with 5–15 mice per group.