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. Author manuscript; available in PMC: 2012 Mar 31.
Published in final edited form as: Nature. 2011 Mar 31;471(7340):637–641. doi: 10.1038/nature09814

Figure 4. Loss of SHARPIN sensitizes cells to FADD- and caspase-8-mediated apoptosis.

Figure 4

a, Increased susceptibility to TNFα-induced apoptosis in SHARPIN deficient MEFs. Cell detachment and death were measured continuously in real-time using an impedance based real-time cell analyzer (RTCA). b, Increased caspase activation by TNFα and CHX in SHARPIN deficient MEFs. c, d, Cell death caused by loss of SHARPIN via FADD and caspase-8 dependent apoptotic signalling. MEFs were retrovirally transduced with indicated proteins and treated with TNFα. e, Increased apoptotic cell death in the skin of Sharpincpdm/Sharpincpdm mice. Normal epidermis (A) and Sharpincpdm/Sharpincpdm (B) skin samples were immunostained for caspase-8 (C), caspase-9 (D) and caspase-3 (E). None of these markers were present in control mouse epidermis (F). H&E; hematoxylin and eosin. Bar = 2 μm. Results in a and c are shown as means and s.e.m. (n=3).