Figure 3. Gene expression profile after differentiated iPSCs was cultured on NF scaffolds in vitro.
(A) The expression of SMC specific markers, including MyoCD, smoothelin, SM22α and SMMHC, was analyzed with quantitative RT-PCR, as shown by indicated times. (B) The expression of pluripotent gene Pou5f1 and other cell lineage specific markers (AFP: endoderm; NeuroD: ectoderm; GATA2: mesoderm). *, p<0.05; **, p<0.01; ***, p<0.001.