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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Cancer Res. 2011 Mar 9;71(9):3400–3409. doi: 10.1158/0008-5472.CAN-10-0965

Figure 1.

Figure 1

Effect of PGI/AMF expression on markers of EMT. Expression of E-cadherin, vimentin, ZEB1, ZEB2 was evaluated by Real-Time RT-PCR in (A) MCF-10A and MDA-MB-231 cells, and (B) BT-549 cells. (C) Effect of PGI/AMF expression on DNA-binding activity of NF-κB. Nuclear proteins were subjected to gel shift assay and densitometric analysis of principal NF-κB bands (marked by arrows) is represented in lower panel. Intensity of NF-κB band in the control cells was taken as 1 and the relative intensities of PGI/AMF-transfected/silenced cells are plotted. (D) Effect of PGI/AMF expression on NF-κB target genes, uPAR and MMP-9 was evaluated by western blot analysis. β-actin protein was used as protein loading control. V, vector control; P/A, PGI/AMF-transfected cells; sP/A, PGI/AMF-silenced cells. **p<0.01 vs. vector control.