Figure 4.
miR-200s influence motility and clonogenicity of breast cells. (A) MCF-10A cells and (B) MDA-MB-231 cells were wounded (time 0h) and maintained for 24h in normal medium. (C) Anchorage-dependent assays for clonogenicity were performed as described under “Methods” section. Bar graphs on the right represent quantification of results presented on the left, in each case. V, vector control; P/A, PGI/AMF-transfected cells; sP/A, PGI/AMF-silenced cells, NP, non-specific pre-miRNA; PM, specific pre-miRNAs (pre-miR200a + pre-miR200b + pre-miR200c); NA, non-specific anti-miRNA; AM, specific anti-miRNAs (anti-miR200a + anti-miR200b + anti-miR200c). *p<0.05 and **p<0.01 vs. non-specific control, NS, non-significant.
