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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Cancer Res. 2011 Mar 9;71(9):3400–3409. doi: 10.1158/0008-5472.CAN-10-0965

Figure 5.

Figure 5

miR-200s modulate PGI/AMF-mediated EMT and invasion of breast cells. (A) Western blot analysis for the expression of indicated EMT markers. β-actin protein was used as protein loading control. PM, specific pre-miRNAs (pre-miR200a + pre-miR200b + pre-miR200c); AM, specific anti-miRNAs (anti-miR200a + anti-miR200b + anti-miR200c); P/A, PGI/AMF-transfected cells; sP/A, PGI/AMF-silenced cells. (B) Invasion of breast cells MCF-10A (upper panel) and MDA-MB-231 (lower panel) was assayed by plating cells in matrigel-coated inserts. The cells that invaded through the matrigel were stained and photographed using fluorescence microscope. a, MCF-10A control cells; b, PGI/AMF- transfected MCF-10A cells; c, PGI/AMF- transfected MCF-10A cells + pre-miR200s (pre-miR200a + pre-miR200b + pre-miR200c); d, MDA-MB-231 control cells; e, PGI/AMF-silenced MDA-MB-231 cells and f, PGI/AMF-silenced MDA-MB-231 cells + anti-miR200s (anti-miR200a + anti-miR200b + anti-miR200c).