Figure 6.
Silencing of PGI/AMF suppresses lung metastasis and anti-miR-200 treatment abrogates this effect. (A) MDA-MB-231 cells (2 × 106 in a total volume of 0.1 ml) were injected via tail vein of ICR-SCID mice, and spontaneous pulmonary metastases were assessed after 5 weeks. The inserts on top of the box plot are representative lungs (left half piece) from each group with arrows indicating grossly visible tumors. (B) H&E staining revealed the extent of metastatic lesions in lungs. V, vector control; sP/A, PGI/AMF-silenced cells, NP, non-specific pre-miRNA; PM, specific pre-miRNAs (pre-miR200s); NA, non-specific anti-miRNA; AM, specific anti-miRNA (anti-miR200b). n=6 mice for each group.
