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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Neurobiol Aging. 2011 Jan 6;33(7):1389–1399. doi: 10.1016/j.neurobiolaging.2010.11.021

Fig. 3.

Fig. 3

Dyrk1A phosphorylates 9G8 in vitro. (a and b) Phosphorylation of 9G8 by Dyrk1A. GST-9G8 or GST (0.2 mg/ml) was incubated with the indicated concentrations of Dyrk1A at 30 °C for 30 min, and the reaction products were separated by SDS-PAGE. Incorporation of 32P into GST-9G8 or GST was detected by autoradiogram (a), and the quantifications are shown in (b). (c) The amino acid sequence of GST-9G8. The red-colored sequences represent those detected by LC-MS/MS. The underlined sequence indicates the 9G8 sequence, which is numbered underneath. (d) Six phospho-peptides from the Dyrk1A-phosphorylated 9G8 samples detected by LC-MS/MS.