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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Cancer Res. 2011 Mar 23;71(9):3246–3256. doi: 10.1158/0008-5472.CAN-10-4058

Figure 4. mTORC1 and mTORC2 regulate epithelial-mesenchymal transition and actin cytoskeletal rearrangement of CRCs.

Figure 4

A. Immunoblot analysis for EMT markers in SW480 cells with sh NTC, sh Raptor and sh Rictor. B. Immunofluorescence staining in SW480 sh NTC, sh Raptor and sh Rictor cells grown in regular growth medium. Bar=50µm. C. Immunoblot analysis in HCT116 and SW480 cells. D. Gelatin zymography of supernatants collected from SW480 sh NTC, sh Raptor and sh Rictor cells stimulated with 5 ng/mL TGFβ for 12 h. E. Apoptosis assessment in HCT116 and SW480 sh NTC, sh Raptor and sh Rictor cells treated with DMSO (control) or Oxaliplatin (10µM) for 24 h (* p<0.05 vs. NTC shRNA).