Effects of presentation rate on ABR magnitude and latency. To challenge stimulus recovery beyond the conventional ABR procedure, the duration of 8-kHz tone bursts was increased to 20 ms and ISI was varied parametrically. A, B Waveforms from a representative mouse in each of the four treatment groups. Numerical labels indicate ISI. C–E Effects of ISI on wave 1 magnitude, interpeak latency (P1 to P2), and wave 2 magnitude. Error bars indicate the inter-quartile range of the distributions. α9KO mice showed statistically smaller wave 1 and 2 magnitudes than quiet-reared WT mice (P < 0.05, asterisks). Noise-reared α9KO mice also showed longer interpeak latencies.