Table 1.
Primer Sequences for Target Genes
| Name | Forward (5′→3′) | Reverse (5′→3′) | Product Size (bp) | Genbank Accession |
|---|---|---|---|---|
| α-CGRP | tgacaggagctaaagctaagtgc | aacatcaacaggatggtttatgg | 142 | NM_001033954 |
| β-AR | caactctgccttcaatcctctta | ctagagtagccgttcccataggt | 117 | NM_007420 |
| 5HT3A | catgtatgccatcctcaacg | ccacgtccacaaactcattg | 188 | BC14479 |
| NOS1 | ctggaggaagtagccaagaaaat | ctcattctccatgtgtttgatga | 177 | NM_008712 |
| NR1 | gtagctgggatcttcctcatttt | gatagccctaaatgtggctttct | 177 | NM_008169 |
| PDP | ttctgaaggctgaagtgataagg | actcgactactgaagagcacagc | 188 | AF026537 |
| PPT-A | atgcagaactacgaaagaagacg | ttcctcatagcgcacattttatt | 110 | NM_009311 |
| TRPV1 | ggctgtcttcatcatcctgttac | ctcttgtgcaatcttgttgacag | 109 | NM_001001445 |
| 18s | aagacgatcagataccgtcgtag | tccgtcaattcctttaagtttca | 160 | NR_003278 |
The information of primer sequences of target genes tested, including gene access number in GenBank, polymerase chain reaction product size (bp). αCGRP: Alpha-calcitonin gene-related peptide; β-AR: β2-adrenergic receptor; 5-HT3A: 5-hydroxytryptamine receptor subunit type 3A; NOS1: Nitric oxide synthase-1; NR1: N-methyl d-aspartate receptor-1; PDP: Prodynorphin; PPT-A: Preprotachykinin-A; TRPV1: transient receptor potential vanilloid-1; 18S: 18S ribosomal RNA gene.