Fig. 3.

GnRH regulates spinophilin expression in hippocampal slice cultures. a Cy3-couples anti-spinophilin was used to detect spinophilin in the CA1 region of hippocampal slice cultures after treatment with GnRH. The staining intensity varied depending on the treatment. The nuclei were counterstained with DAPI (blue; scale bar 20 μm). b Image analysis of IHC for the postsynaptic protein spinophilin in hippocampal slice cultures after 8 days of GnRH treatment demonstrated that an intermediate dose of GnRH (10 nM) resulted in a significant increase in the staining index compared to the control. The staining index was significantly lower after treatment with 500 nM GnRH and was not significantly altered compared to the control (mean ± SEM, n = 10)