Fig. 4.
Effects of GnRH on spinophilin expression is mediated by estrogen synthesis in hippocampal dispersion cultures. a Punctate spinophilin immunoreactivity was detected on single pyramidal neurons (red, Cy3). The stained area and its staining intensity varied with the different treatments. The nuclei were counterstained with DAPI (blue), scale bar 10 μm. b The GnRH-induced increase of spinophilin expression was blocked when E2 synthesis was inhibited by letrozole (10−7 M). In contrast to the treatment with GnRH alone, the combination of GnRH (10−8 M) and letrozole (10−7 M) did not result in an increase in the spinophilin staining (mean ± SEM, n = 5)