Table 1.
Identified proteins with preload-dependent changes of spot intensity
| Spot no. | Identification | Accession no. | Theoretical pI/mass | Observed range of pI/mass | Ratio preloaded/unloaded (P value) | Ratio preloadedCysA/unloadedCysA (P values) |
|---|---|---|---|---|---|---|
| 1 | (CH60_MOUSE) 60 kDa heat shock protein, mitochondrial precursor (Hsp60) | P63038 | 5.5/61.0 | 5.0–5.4/37–66 | 1.21 (ns) | 1.88 (0.03) |
| 2 | (ODPB_HUMAN) Pyruvate dehydrogenase E1 component subunit beta | P11177 | 5.4/36.8 | 4.5–5.0/31–37 | 3.73 (0.045) | 0.78 (ns) |
| 3 | (UQCR1_MOUSE) Ubiquinol-cytochrome-c reductase complex core protein I, mitochondrial precursor | Q9CZ13 | 5.7/52.7 | 5.0–5.4/37–66 | 3.10 (0.048) | 0.79 (ns) |
| 4 | (TPIS_RABIT) Triosephosphate isomerase | P00939 | 7.0/26.7 | 6.0–8.5/17.5–31 | 5.98 (0.008) | 0.86 (ns) |
| 5 | (TPIS_RABIT) Triosephosphate isomerase | P00939 | 7.0/26.7 | 6.0–8.5/17.5–31 | 3.81 (0.007) | 1.12 (ns) |
| 6 | (ACON_PIG) Aconitate hydratase, mitochondrial precursor | P16276 | 8.2/85.7 | 6.0–8.5/>76 | 7.48 (0.014) | 0.94 (ns) |
| Spot no. | Identification | Accession no. | Theoretical pI/mass | Observed range of pI/mass | Ratio preloaded/unloaded (P value) | Ratio preloadedCysA/unloadedCysA (P value) |
|---|---|---|---|---|---|---|
| 7 | (ALDOA_RABIT) Fructose-bisphosphate aldolase A (Muscle-type aldolase) | P00883 | 8.4/39.2 | 6.0–8.5/37–66 | 5.08 (0.006) | 0.85 (ns) |
| 8 | (ALDOA_RABIT) Fructose-bisphosphate aldolase A (Muscle-type aldolase) | P00883 | 8.4/39.2 | 8.5–10/37–66 | 5.04 (0.025) | 0.87 (ns) |
| 9 | (ATPA_MOUSE) ATP synthase alpha chain, mitochondrial precursor ATP | Q03265 | 7.52/56.5 | 6.0–8.5/37–66 | 4.16 (0.007) | 1.12 (ns) |
| 10 | (ATPA1_BOVIN) ATP synthase alpha chain heart isoform mitochondrial precursor | P19483 | 7.52/56.5 | 6.0–8.5/37–66 | 5.98 (0.049) | 0.93 (ns) |
| Spot no. | Identification | Accession no. | Theoretical pI/mass | Observed range of pI/mass | Ratio preloaded/unloaded (P value) | Ratio preloadedCysA/unloadedCysA (P value) |
|---|---|---|---|---|---|---|
| 11 | (HCDH_PIG) Short chain 3-hydroxyacyl-CoA dehydrogenase, mitochondrial precursor | P00348 | 7.6/33.3 | 8.5–10.0/31–37 | 10.0 (0.006) | 1.25 (ns) |
| 12 | (NUBM_MOUSE) NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial precursor | Q91YT0 | 8.4/50.6 | 6.0–8.5/37–66 | 3.21 (0.019) | 1.47 (ns) |
| 13 | (NUBM_MOUSE) NADH-ubiquinone oxidoreductase 51 kDa subunit, mitochondrial precursor | Q91YT0 | 8.4/50.6 | 6.0–8.5/37–66 | 4.41 (0.048) | 1.32 (ns) |
| 14 | (KPYK_XENLA) Pyruvate kinase muscle isozyme | Q92122 | 6.5/57.0 | 6.0–8.5/37–66 | 6.28 (0.014) | 0.88 (ns) |
| 15 | (END8_ECO57) Endonuclease VIII | Q8X9C6 | 8.12/29.6 | 6.0–8.5/17.5–31 | 3.16 (0.009) | 1.22 (ns) |
| Spot no. | Identification | Accession no. | Theoretical pI/mass | Observed range of pI/mass | Ratio preloaded/unloaded (P value) | Ratio preloadedCysA/unloadedCysA (P value) |
|---|---|---|---|---|---|---|
| 16 | (ETFA_MOUSE) Electron transfer flavoprotein alpha-subunit, mitochondrial precursor | Q99LC5 | 5.7/35.0 | 5.4–6.0/31–37 | 2.91 (0.046) | 0.77 (ns) |
| 17 | (HUTH_RHIME) histidine ammonia-lyase | O31197 | 5.7/52.7 | 5.4–6.0/37–66 | 0.83 (ns) | 0.27 (0.004) |
Protein identification after tryptic in-gel digest with ESI-MS/MS mass spectrometry and database search. Spot numbers correspond to Figs. 2, 3, 4 of the manuscript and tables of the online supplement. Theoretical molecular mass is in kilo dalton (kDa) and isoelectric point (pI) according to the ExPASY database. Observed mass and pI of identified proteins on 2-DE by using a mass/pI protein standard from 17.5–76 kDa with a pI range from 4.5–8.5 (2-D SDS-PAGE protein standards; Bio-Rad). Ratio preloaded/unloaded and ratio preloadedCysA/unloadedCysA represent the change of spot intensity in these different experimental groups. Significantly changed spot intensities are given in bold letters with the level of statistical significance in parenthesis. For detailed data of spot intensities see the online supplement.